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Fig. 3 | Virology Journal

Fig. 3

From: Variable 3’polyadenylation of Wheat yellow mosaic virus and its novel effects on translation and replication

Fig. 3

Effect of polyadenylation on in vitro translation mediated by WYMV UTRs. a Constructs of firefly luciferase reporter vector containing UTRs of WYMV RNA1. b Effect of UTRs of WYMV RNA1 with or without poly(A) on translation of F-luc. c Constructs of firefly luciferase reporter vector containing UTRs of WYMV RNA2. d Effect of UTRs of WYMV RNA2 with or without poly(A) on translation of F-luc. 5’UTR and 3’UTR of WYMV RNA1 (AF067124) is 162 nt and 258 nt, respectively; 5’UTR and 3’UTR of WYMV RNA2 (AF041041) is 171 nt and 768 nt, respectively. F-luc indicates firefly luciferase coding region; shadow boxes indicate corresponding WYMV UTRs

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Virology Journal

ISSN: 1743-422X