Fig. 3
From: Regulation of cyclin T1 during HIV replication and latency establishment in human memory CD4 T cells
Generation of p24+CycT1- and p24+CycT1+ cells during HIV replication in memory CD4 T cells. Memory CD4 T cells were purified from peripheral blood, then uninfected or infected with HIV (R5 strain NSN-SX) in IL2 medium for 2 days. Cells were washed and cultured with CD3+CD28 mabs and IL2 for 7 days. Cells were then stained for CycT1 and intracellular p24. a-b Shown are sample dotplots and mean ± sem p24/CycT1 expression during HIV replication (N = 3–4). c Activation (CD69 expression) and viability levels of uninfected CycT1+ and infected p24+CycT1- and p24+CycT1+ memory CD4 T cells (*p < 0.05)