Fig. 2

CycT1 expression and HIV production during cell cycle progression of memory CD4 T cells. Memory CD4 T cells were uninfected or HIV-infected (R5 strain SF162) in IL2 medium for 2 days, washed, and cultured for 5 days with CD3+CD28 costimulation and IL2 or IL2 alone. Cells were then stained with propidium iodide for DNA content in conjunction with either CycT1-FITC or p24-FITC abs. a Sample cell cycle distributions and CycT1 expression in G1, S, or G2 phases of uninfected and HIV-infected memory CD4 T cells after 5 days costimulation. b Sample cell cycle distributions and intracellular p24 levels in G1, S, or G2 phases of HIV-infected memory CD4 T cells after 5 days costimulation. c Mean ± sem CycT1 and p24 levels during cell cycle progression of memory CD4 T cells (*p < 0.05, N = 3)