Fig. 1
From: Regulation of cyclin T1 during HIV replication and latency establishment in human memory CD4 T cells
Assessment of CycT1 expression in uninfected memory CD4 T cells during T cell activation. Human CD4+CD45RO+ memory T cells were purified from peripheral blood and cultured without (No Costimulation) or with CD3+CD28 mabs and IL2 (Costimulation) for 1–5 days. Cells were then stained for CycT1, CD69, CD69, HLA.DR, and CD38. a Shown are sample flow cytometry dotplots of CD69/CD25 and HLA.DR/CD38 expression of memory CD4 T cells without or with costimulation, and (b) overlays of CycT1 expression. c Mean ± sem CycT1 expression gated on different CD69/CD25 and HLA.DR/CD38 populations (*p < 0.05, N = 3–4)