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Table 1 Subjective scale for semi-quantitative analysis of the degree of liver injury based on histopathological alterations

From: Efficacy of geraniin on dengue virus type-2 infected BALB/c mice

Histopathological alterations

0

1

2

3

Hydropic degeneration (hepatocyte swelling)

None

Mild (small area, ground glass appearance of hepatocytes)

Moderate (larger area, ground glass appearance of hepatocytes)

Diffuse (feathery appearance of hepatocytes)

Necrosis & haemorrhage

None

Mild (no haemorrhage)

Moderate (small area, focal)

Severe (large area, multifocal)

Venous outflow congestion (dilatation of sinusoids)

None

Mild (slight)

Moderate (focal)

Severe (diffuse)

Steatosis

None

Mild (small area, microsteatosis)

Moderate (larger zones, micro- and macrosteatosis)

Severe (diffuse micro- and macrosteatosis)

Pyknotic nucleus

None

Mild

Moderate

Severe

  1. Liver sections from six replicates mice (n = 6) were evaluated using a scale ranging from 0 to 3 (0 = none, 1 = mild, 2 = moderate and 3 = severe) according to the degree of hydropic change (hepatocyte swelling); necrosis and haemorrhage; venous outflow congestion (dilatation of sinusoids); steatosis; and pyknotic nucleus