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Fig. 4 | Virology Journal

Fig. 4

From: Functional analysis of apple stem pitting virus coat protein variants

Fig. 4

ASPV CPs possess VSR activity. Local patches on N. benthamiana leaf were agroinfiltrated with 35S:mGFP5 in combination with either 35S:P19, 35S:P25, PVX (wt) or PVX-ASPV-CPs, respectively. a PVX-ASPV-CPs were infiltrated on the same leaf. b PVX-ASPV-CPs were infiltrated on different leaves. GFP signal was monitored by UV illumination at 4 days post infiltration (dpi) (a) and 6 dpi (b). c Total protein was extracted from leaf infiltration spots of (b). Samples were subjected to anti-GFP immune-blotting. Ponceau staining are shown as a loading control. All experiments were repeated 3 times and representative results are shown

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