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Fig. 1 | Virology Journal

Fig. 1

From: Construction and characterization of an infectious cDNA clone of potato virus S developed from selected populations that survived genetic bottlenecks

Fig. 1

Schematic representation of the genome and full-length cDNA clones of PVS isolate H95. PVS-H95 genome organization is shown at the top. The start and stop positions of each ORF (rectangle) are indicated with nucleotide numbers. Nucleotide substitutions among the eight full-length cDNA clones of PVS-H95 (bottom) are indicated with colored arrowheads; numbers indicate the nucleotide positions. A full-length cDNA clone pPVS-H-FL-AB was constructed by connecting eight clones (colored rectangles). Arrow indicates T7 promoter. Seven additional clones were constructed either by the replacement of a fragment produced by site-directed mutagenesis (e.g., pPVS-H-FL-V, −D, −E, -F) or from a different clone (e.g., pPVS-H-FL-C, −G, -H). The cDNA clone pPVS-H-FL-H with an asterisk repesents the consensus sequence of PVS-H95 genome. Nucleotides and coding amino acids in parentheses with a gray background vary from the consensus sequence of PVS-H95 genome. Genomic sequence comparison between PVS-H95 (LC375227) and PVS-H00 (pPVS-H-FL-β, LC375228) is summarized in Table 2, Fig. 5 and Additional file 2: Figure S1

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