Fig. 5

Comparison of the accumulation level of potato virus X (PVX) RNAs in Solanum lycopersicum (cv. Beta Lux). The analyzed plants were inoculated with PVX-empty construct and PVX chimeras, PVX-Vp23, PVX-Vp26 and PVX-Vp35. a Accumulation of PVX genomic RNA (RNA-dependent RNA polymerase - RdRP) was analyzed by menas of quantitative real-time RT-PCR. The RNA used for the analysis was extracted from systemic leaves of tomato seedlings 14 days post infiltration. The copy number of PVX genomic RNA per 50 ng of total RNA was displayed. The mean (the x), the median (the central horizontal bar) and the outliers (the dots) were included in the box charts. b Western blot analysis of PVX coat protein (CP) accumulation in S. lycopersicum inoculated with tested PVX variants. The normalized densitometry quantitation of chemiluminescence signal was included. The equal protein load was assessed by Coomassie brilliant blue (CBB) staining