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Fig. 4 | Virology Journal

Fig. 4

From: Contribution of Tomato torrado virus Vp26 coat protein subunit to systemic necrosis induction and virus infectivity in Solanum lycopersicum

Fig. 4

Detection of recombinant potato virus X-Vps in infected tomato plants. Total RNA was extracted from systemic leaves of plants infected with each PVX-Vp individually (14 days post infiltration) and taken for reverse transcriptase polymerase chain reaction (RT-PCR) with corresponding Vp-specific primers. Specific RT-PCR products were detected for Vp23 (650 base pairs, bp), Vp26 (710 bp) and Vp35 (737 bp) in plants infected with PVX-Vp23, PVX-Vp26 and PVX-Vp35, respectively. Primers flanking a multi-cloning site (mcs) of PVX-vector gave a product of ca. 250 bp for the empty vector

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