Fig. 6
From: Involvement of PRRSV NSP3 and NSP5 in the autophagy process
The NSP3 hydrophilic domain is required to activate autophagy. a Schematic of the PRRSV NSP3, NSP5 and mutant NSP3 protein structures. b mCherry was detected by immunoblotting in Nsp3- and mutant Nsp3-expressing cells c Marc-145 cells were co-transfected with plasmids expressing wildtype/mutant PRRSV Nsp3 and GFP-LC3 for 24 h. Fixed cells were detected using fluorescence microscopy. GFP-LC3 puncta represent mature autophagosomes; nuclei were stained with DAPI (blue). Scale bars: 10 μm