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Fig. 5 | Virology Journal

Fig. 5

From: Involvement of PRRSV NSP3 and NSP5 in the autophagy process

Fig. 5

a NSP3 and NSP5 were located in the ER but not the mitochondria. Nsp3-mCherry and Nsp5-mCherry were expressed in Marc-145 cells; the ER was stained with an antibody against endogenous calnexin (green), and mitochondria were stained with MitoTracker Green. Scale bars: 10 μm. b Statistical analysis of the Pearson correlation coefficients for calnexin/MitoTracker and NSP3/NSP5. The colocalization coefficient is presented as the ratio of fluorescent signals of calnexin that were positive for NSP3/NSP5. The colocalization coefficient is the ratio of fluorescent signals of calnexin that were negative for NSP3/NSP5. c CHOP expression was examined in mock-infected; PRRSV-infected; and mCherry-, Nsp3-mCherry-, or Nsp5-mCherry-expressing Marc-145 cells using immunoblotting. The intensities of CHOP bands were normalized to GAPDH. The splicing of the Xbp1 mRNA was examined using PCR; Xbp1u is the unspliced mRNA, and Xbp1s is the spliced mRNA

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