Fig. 4

Lysosome fusion with autophagosomes induced by NSP3 and NSP5. a Marc-145 cells were starved for 4 h or infected with PRRSV CH-1a for 24 h, and the endogenous LC3 and LAMP1 proteins were labeled with green and red fluorophores, respectively. Scale bars: 10 μm. Statistical analysis of the Pearson correlation coefficient for LC3 and LAMP1. The colocalization coefficient is presented as the ratio of fluorescent signals of LC3 that were positive for LAMP1. b Nsp3-mCherry or Nsp5-mCherry was transfected into cells for 24 h. Cells were fixed, stained with fluorescent dye-conjugated LC3 and LAMP1 antibodies, and observed under a fluorescence microscope. Scale bars: 10 μm. Statistical analysis of the Pearson correlation coefficient for LC3 and LAMP1. The colocalization coefficient is presented as the ratio of fluorescent signals of LC3 that were negative for LAMP1