Fig. 3

Autophagosomes originated from the ER but not mitochondria. a Marc-145 cells were transfected with Nsp3-mCherry and Nsp5-mCherry for 24 h; ATG5 is labeled in green, and nuclei were stained with DAPI (blue). Scale bars: 10 μm. Statistical analysis of the Pearson correlation coefficient for calnexin and ATG5. The Pearson colocalization coefficient is presented as the ratio of punctate signals of calnexin that were positive for ATG5. b Nsp3-mCherry and Nsp5-mCherry were expressed in Marc-145 cells. Twenty-four hours after transfection, cells were cultured with MitoTracker Green, a marker of mitochondria, and were then fixed and immunostained with an anti-ATG5 antibody (pink). Nuclei were stained with DAPI (blue). Scale bars: 10 μm. Statistical analysis of the Pearson correlation coefficient for MitoTracker Green and ATG5. The colocalization coefficient is presented as the ratio of fluorescent signals of MitoTracker Green that were negative for ATG5