Development of a duplex SYBR Green I-based quantitative real-time PCR assay for the rapid differentiation of goose and Muscovy duck parvoviruses

Table 1 Variance analysis of Ct values for real-time PCR assay

Concentration of standard plasmid (DNA copies/μL)	Intra-assay variability			Inter-assay variability
	CT		CV (%)	CT		CV (%)
	Mean	SD	CV (%)	Mean	SD	CV (%)
GPV
1 × 10⁸	10.72	0.17	1.59	10.64	0.16	1.51
1 × 10⁷	14.60	0.45	3.05	14.39	0.32	2.24
1 × 10⁶	17.47	0.15	0.87	17.13	0.62	3.60
MDPV
1 × 10⁸	13.31	0.12	0.92	13.18	0.19	1.47
1 × 10⁷	16.04	0.35	2.21	15.99	0.21	1.29
1 × 10⁶	18.02	0.08	0.44	17.93	0.31	1.73

ISSN: 1743-422X