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Table 1 Variance analysis of Ct values for real-time PCR assay

From: Development of a duplex SYBR Green I-based quantitative real-time PCR assay for the rapid differentiation of goose and Muscovy duck parvoviruses

Concentration of standard plasmid (DNA copies/μL) Intra-assay variability Inter-assay variability
CT CV (%) CT CV (%)
Mean SD Mean SD
GPV
 1 × 108 10.72 0.17 1.59 10.64 0.16 1.51
 1 × 107 14.60 0.45 3.05 14.39 0.32 2.24
 1 × 106 17.47 0.15 0.87 17.13 0.62 3.60
MDPV
 1 × 108 13.31 0.12 0.92 13.18 0.19 1.47
 1 × 107 16.04 0.35 2.21 15.99 0.21 1.29
 1 × 106 18.02 0.08 0.44 17.93 0.31 1.73