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Fig. 5 | Virology Journal

Fig. 5

From: Optimization of Zika virus envelope protein production for ELISA and correlation of antibody titers with virus neutralization in Mexican patients from an arbovirus endemic region

Fig. 5

ZIKV PRNT assays assessing the level of neutralising antibodies in human sera from ZIKV infected patients. a. ZIKV preparation for the neutralization assay was subjected to western-blot under reducing and non-reducing conditions. Different volumes (ul) were loaded in decreasing volumes and bands detected by an anti-ZIKV specific antibody. Positive control is ZIKV Env-CD4. b. ZIKV ND50 titers for sera from ZIKV infected patients and control sera. Sera from Zika patients (blue) and sera obtained from healthy donors (who should have less or no previous flaviviral exposure) (red) c. Correlation between the endpoint reciprocal titers from the ELISA assay and ND50 titers from the PRNT assay for ZIKV patients compared to the healthy donors. P values and R2 values reflect Pearson correlation tests

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