Table 1 Calculated presented challenge dose of EEEV (Pe-6) to groups of female Balb/c mice, with subsequent survival and clinical data. Each aerosol challenge utilised 10 mL of fresh virus preparations, in a 10 mins nose-only aerosol exposure
INPUT Collison (pfu/ml) | OUTPUT Impinger (pfu/ml) | Calc. Presented Dose (pfu/mouse) | Survival (%) | Mean Time to Death (days) | Max. mean weight loss (%) a, b | Max. mean clinical score a, b | Max. viral load in brain (Log10 pfu/g)c |
|---|---|---|---|---|---|---|---|
0.0E+ 00 | 0.0E+ 00 | MOCK | 8/8 (100) | – | 1.2, n/a | 0.0, n/a | – |
1.6E+ 02 | 0.0E+ 00 | 0.0E+ 00 | 8/8 (100) | – | 0.1, n/a | 0.0, n/a | – |
1.8E+ 03 | 6.0E+ 00 | 1.0E+ 00 | 8/8 (100) | – | 0.6, n/a | 0.0, n/a | – |
2.3E+ 04 | 4.4E+ 01 | 7.0E+ 00 | 8/8 (100) | – | 1.0, n/a | 0.0, n/a | – |
1.9E+ 05 | 1.4E+ 02 | 2.4E+ 01 | 7/8 (88) | 3.1 | 2.0, 11.6 | 0.5, 4.0 | 9.9 (n = 1) |
3.1E+ 06 | 2.5E+ 03 | 4.3E+ 02 | 3/8 (38) | 4.2 | 6.4, 13.3 | 3.3, 8.0 | 9.7 (n = 3) |
2.0E+ 08 | 1.7E+ 04 | 2.8E+ 03 | 0/8 (0) | 3.0 | 12.9, 12.9 | 4.6, 4.6 | 9.5 (n = 5) |