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Table 1 Calculated presented challenge dose of EEEV (Pe-6) to groups of female Balb/c mice, with subsequent survival and clinical data. Each aerosol challenge utilised 10 mL of fresh virus preparations, in a 10 mins nose-only aerosol exposure

From: Aerosol infection of Balb/c mice with eastern equine encephalitis virus; susceptibility and lethality

INPUT Collison (pfu/ml) OUTPUT Impinger (pfu/ml) Calc. Presented Dose (pfu/mouse) Survival (%) Mean Time to Death (days) Max. mean weight loss (%) a, b Max. mean clinical score a, b Max. viral load in brain (Log10 pfu/g)c
0.0E+ 00 0.0E+ 00 MOCK 8/8 (100) 1.2, n/a 0.0, n/a
1.6E+ 02 0.0E+ 00 0.0E+ 00 8/8 (100) 0.1, n/a 0.0, n/a
1.8E+ 03 6.0E+ 00 1.0E+ 00 8/8 (100) 0.6, n/a 0.0, n/a
2.3E+ 04 4.4E+ 01 7.0E+ 00 8/8 (100) 1.0, n/a 0.0, n/a
1.9E+ 05 1.4E+ 02 2.4E+ 01 7/8 (88) 3.1 2.0, 11.6 0.5, 4.0 9.9 (n = 1)
3.1E+ 06 2.5E+ 03 4.3E+ 02 3/8 (38) 4.2 6.4, 13.3 3.3, 8.0 9.7 (n = 3)
2.0E+ 08 1.7E+ 04 2.8E+ 03 0/8 (0) 3.0 12.9, 12.9 4.6, 4.6 9.5 (n = 5)
  1. aMean values obtained for all mice in the group regardless of clinical condition
  2. bMean values obtained for mice observed to have observable or pronounced clinical signs
  3. cValues obtained from brains excised on the day mice succumbed to disease (i.e. day 3–4 post challenge)