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Fig. 1 | Virology Journal

Fig. 1

From: Crystal structure of Usutu virus envelope protein in the pre-fusion state

Fig. 1

Expression and purification of the USUV-E protein. a A schematic view of USUV-E. The ecto-domain and the transmembrane domains (TM) of the protein are indicated. For USUV-E preparation, its ecto-domain region spanning residues 1–406 was expressed as a fusion protein with a C-terminal 6xHis tag. The three domains of this ecto-domain protein are highlighted in red, yellow, and blue, respectively, and the fusion loop is in grey. b Small-scale expression and inclusion-body extraction. The SDS-PAGE results are shown. Lane 1, un-induced; lane 2, induction with 0.2 mM IPTG; lane 3, induction with 1 mM IPTG; lane 4, the extracted inclusion bodies. The USUV-E protein was marked with arrows. c Purification of the refolded USUV-E protein by size exclusion chromatography. The separation chromatograph of the protein and the SDS-PAGE analyses of the pooled samples are shown

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