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Fig. 2 | Virology Journal

Fig. 2

From: The eukaryotic translation elongation factor 1A regulation of actin stress fibers is important for infectious RSV production

Fig. 2

Did B treatment downregulates actin stress fiber formation in HEp-2 cells. HEp-2 cells were treated with 2.5 nM Did B, DMSO or left untreated. a Uninfected and (b) RSV infected were stained 48 h later to show (a and b) F-actin using Alexa Fluor 647 phalloidin or (b) RSV protein using a anti-RSV antibody. The stained cells were visualised by confocal microscopy and a maximum intensity projection is shown. a In Did B treated cells, examples of F-actin accumulation at the cell cortex are boxed. a and b Actin stress fibers, as exampled by circles, in control HEp-2 cells that are sharply downregulated in Did B treated cells. b, (bottom row) The merged images of Alexa Fluor 647 phalloidin and FITC staining are shown

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