Table 1 Real-time PCR primers sequences

TLR1

Sense: CAGTGTCTGGTACACGCATGGT;

Antisense: TTTCAAAAACCGTGTCTGTTAAGAGA;

TLR2

Sense: GCCTCTCCAAGGAAGAATCC;

Antisense: TCCTGTTGTTGGACAGGTCA;

TLR3

Sense: TGGTTGGGCCACCTAGAAGTA;

Antisense: TCTCCATTCCTGGCCTGTG;

TLR4

Sense: AAGCCGAAAGGTGATTGTTG;

Antisense: CTGAGCAGGGTCTTCTCCAC;

TLR5

Sense: TGCCTTGAAGCCTTCAGTTATG;

Antisense: CCAACCACCACCATGATGAG;

TLR6

Sense: GAAGAAGAACAACCCTTTAGGATAGC;

Antisense: AGGCAAACAAAATGGAAGCTT;

TLR7

Sense: TTTACCTGGATGGAAACCAGCTA;

Antisense: TCAAGGCTGAGAAGCTGTAAGCTA;

TLR8

Sense: TTATGTGTTCCAGGAACTCAGAGAA;

Antisense: TAATACCCAAGTTGATAGTCGATAAGTTTG;

TLR9

Sense: GGACCTCTGGTACTGCTTCCA;

Antisense: AAGCTCGTTGTACACCCAGTCT;

MD2

Sense: CCGATGCAAGTATTTCATACACCTACT;

Antisense: CTCCTTGGAATGTAGAAAATGTGC;

GAPDH

Sense: TGCACCACCAACTGCTTAGC;

Antisense: GGCATGGACTGTGGTCATGAG;