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Fig. 7 | Virology Journal

Fig. 7

From: Herpes simplex virus type 2 infection triggers AP-1 transcription activity through TLR4 signaling in genital epithelial cells

Fig. 7

Cellular AP-1 activation might be essential for TLR4 promoter-driven transcription. a SP600125 could inhibit HSV-2-induced AP-1 activation. HEC-1-A cells were transfected with TLR4-promoter-luc plasmid. Cells were mock-treated or treated with SP600125 (10 μM), and then infected with HSV-2 (moi = 1) 24 h post-transfection. The relative luminescence units were determined 24 h p.i. b AP-1 transcriptional factor binding site was important for TLR4 promoter activity. HEC-1-A cells were transfected with TLR4-promoter-luc, and its truncated promoter luciferase reporter plasmid. After 24 h, cells were infected with HSV-2 (moi = 1) and the relative luminescence units were determined 24 h p.i. All experiments were performed three times, and the representative results were shown

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