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Fig. 5 | Virology Journal

Fig. 5

From: Herpes simplex virus type 2 infection triggers AP-1 transcription activity through TLR4 signaling in genital epithelial cells

Fig. 5

HSV-2 infection up-regulated cell membrane-associated TLR4 expression. a The distribution of TLR4 in cytoplasm and membrane of U937 cells. U937 cells were harvested and cytoplasmic or membrane-associated total proteins were exacted as described. TLR4 expression in each component was determined via western blot. MEK1/2 was set as the control for monitoring cytoplasm/membrane fractionation quality. b HSV-2 infection increased membrane-anchoring TLR4 content. HEC-1-A cells were mock-infected or infected with HSV-2 (moi = 1). Cells were harvested and cytoplasmic or membrane-associated total proteins were exacted as describe 24 h p.i. TLR4 expressions were examined via western blot. c LPS could enhance HSV-2-induced AP-1 activation. HEC-1-A cells were transfected with AP-1-luc plasmid. Cells were mock-infected or infected with HSV-2 (moi = 1) in the absence or presence of serial concentrations of LPS 24 h post-tranfection. Relative luminescence units were determined as described. All experiments were performed twice, and the representative results were shown

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