Fig. 4

Overexpression of TLR4, MD2 or both augmented HSV-2-mediated AP-1 activation in genital epithelial cells. a MD2 expression was determined in 4 different genital epithelial cell lines via RT-PCR. b HSV-2 infection did not affect MD2 mRNA expression in HEC-1-A cells (a). Total RNA was extracted from HEC-1-A cells at time points as described. TLR4 or MD2 mRNA transcription level was determined through real-time PCR. c HEC-1-A cells were co-tranfected with TLR4, MD2 or both expression vectors, and pAP-1-luc and pRL-TK plasmids. Cells were mock-infected or infected with HSV-2 (moi = 1) 24 h post-transfection. RLUs were determined as described. All experiments were performed three times. The representative results were shown