Fig. 1

The profiles of TLRs expression in the human genital epithelial cells and their expression level regulated by HSV-2 infection. a TLRs expression profiles in HEC-1-A and VK2 cells. Total RNA was extracted from HEC-1-A or VK2 cells, and TLRs were detected via RT-PCR. b–c certain TLRs expression fold change after HSV-2 infection in HEC-1-A cells (b) and VK2 (c). Cells were harvested 6 h or 24 h p.i. and total RNA was extracted as described. TLRs expression was detected via real-time PCR. d TLR4 mRNA expression in HEC-1-A and 3 normal human genital epithelial cells with different anatomical positions. Total RNA were extracted from the different cell lines as described, and TLR4 expression were evaluated via RT-PCR. All experiments were performed three times and the representative experiment results were shown