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Fig. 4 | Virology Journal

Fig. 4

From: Human papillomavirus type 16 E6 and E7 oncoproteins interact with the nuclear p53-binding protein 1 in an in vitro reconstructed 3D epithelium: new insights for the virus-induced DNA damage response

Fig. 4

Double immunofluorescent analysis of E6 and E7 with cyclin E2 and B1. E6 and E7 HPV16 pushes a subset of differentiating cells into G1/S (cyclin E2 positive cells, B and F) and S/G2 checkpoint phases (cyclin B1 positive cells, C and G). Double immunofluorescent analysis of E6 and E7 with 53BP1. CaSki cells have a high number of discrete small 53BP1 positive nuclear foci inside most of the same cells that were E6 (A-A’-A”) and E7 (E, E’, E”) positive; in HPV16 epithelial models E6/53BP1 (D) and E7/53BP1 (H) positivity is throughout the epithelium, both in undifferentiated and differentiated layers. Nuclei were always visualized in blue with DAPI counterstaining. Bar scales in 20x fields = 100 μm

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