Fig. 1

H&E staining. A) Control in vitro reconstructed 3D epithelial culture made up with normal keratinocytes and B) E6E7 HPV16 transduced keratinocytes. C) In vivo normal epithelia obtained from tumor-free mucosa margins. D) HPV16 positive anal intraepithelial neoplasia (AIN), grade III. Bar scales in 20x fields = 100 μm