Table 1 Primers used for HEV quantification, genotyping, and complete genome sequencing
From: A new hepatitis E virus genotype 2 strain identified from an outbreak in Nigeria, 2017
Primera | Sequence (5′-3′) | Locationb | Use | Reference |
|---|---|---|---|---|
HEV-07_f | GGTGGTTTCTGGGGTGAC | 5261–5278 | HEV-1 to HEV-4 quantification | [18] |
HEV-TM3_f | FAM-TGATTCTCAGCCCTTCGC-MGB | 5284–5301 | ||
HEV-08_r | AGGGGTTGGTTGGATGAA | 5330–5313 | ||
HEV-38_f | GARGCYATGGTBGAGAARG | 4084–4102 | HEV-1 to HEV-4 genotyping in ORF1 | [18] |
HEV-39_r | GCCATRTTCCARACRGTRTTCC | 4622–4601 | ||
HEV-37_f | GGTTYCGYGCYATTGARAARG | 4277–4297 | ||
HEV-27_r | TCRCCRGARTGYTTCTTCC | 4583–4565 | ||
HEV-30_f | CCGACAGAATTRATTTCGTCGG | 6296–6317 | HEV-1 to HEV-4 genotyping in ORF2 | |
HEV-32_f | GTCTCRGCCAATGGCGAGCCRRC | 6350–6372 | ||
HEV-31_r | GTYTTRGARTACTGCTGR | 6750–6733 | ||
HEV-266_f | GCARGCTGCTCTRGCWGCGGC | 78–98 | HEV-2 complete genome sequencing | This study |
HEV-274_f | TGGTGGTTAGGCCTTTTCTCTC | 122–143 | ||
HEV-275_f | CCGATCCAGCGTGTCATACATA | 223–244 | ||
HEV-267_r | GGRGCWGWRTACCARCGCTG | 392–373 | ||
HEV-268_f | AYCTYCGYGGYATTAGCTAYAAGG | 1055–1078 | ||
HEV-276_r | CGTTGATGGCAAATTGTGAGGT | 1178–1157 | ||
HEV-277_f | ATCTCTCGTCTCTACAGCTGGT | 1246–1267 | ||
HEV-278_f | GGGCCGTCAGTTGCAATTTTAT | 1299–1320 | ||
HEV-283_f | GTAGCTGCCGGACTATTGCT | 1397–1416 | ||
HEV-270_r | ARCCACYKCATAAARCARC | 1457–1439 | ||
HEV-284_f | ACCAGGGCCATGACAATGAG | 1508–1527 | ||
HEV-285_r | GAGGCCTGGTCAGCAACTAG | 2186–2164 | ||
HEV-271_f | AACCCMAAGAGGCUYGAGGC | 2620–2639 | ||
HEV-272_f | GCCTGGGARCGKAAYCAYCG | 2734–2753 | ||
HEV-279_f | TGTTCAACGTAGGATGATCCGG | 2833–2812 | ||
HEV-280_f | TTTGAGCATACTGGTCTGGTCC | 3220–3241 | ||
HEV-273_r | CARCGRUGKGURACAUGCCACC | 3296–3275 | ||
HEV-235_r | CYGCCTGGGTGAACACTAG | 3421–3403 | ||
HEV-265_f | ATGGGGACGCCTATGATGAATC | 4337–4358 | ||
HEV-282_r | TTCTGGGTCGAGTCAAACTCAG | 4439–4418 | ||
HEV-281_r | CACTCCTCCATAATAGCGCACT | 4481–4460 | ||
HEV-286_f | TTCTGCTGTTGCTCCTCCTG | 5169–5188 | ||
HEV-301_f | AGACGTCTGGTGTTGCTGAG | 5937–5956 | ||
HEV-288_r | TTTACTGTCGGCTCGGCATT | 6384–6365 | ||
HEV-287_r | GCTGGGCATTCTCCACAGAT | 6413–6394 | ||
HEV-233_f | GCCTSTTTTGTGATGCGCG | 6755–6773 |