Skip to main content

Advertisement

Fig. 6 | Virology Journal

Fig. 6

From: Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway

Fig. 6

Wee1 and Myt1 expression and Cdc25C phosphorylation enhance after PRRSV infection. a Wee1 and Myt1 expression in mock-and PRRSV-infected MARC-145 cells. Cell lysates were collected at the indicated time points post-infection, and the expression of Wee1 and Myt1 was determined by western blot. MARC-145 cells treated with 50 ng/mL Noco. for 16 h served as a positive control (left). Wee1 and Myt1 expression levels were quantitatively analyzed and compared with GAPDH expression level using Image J (right). *** indicates p < 0.001. b PRRSV infection induced phosphorylation of Cdc25C in infected MARC-145 cells. Lysates from mock-and PRRSV-infected cells were prepared at the indicated time points and processed for western blot with specific antibodies against Cdc25C and phospho-Cdc25C (Ser216). MARC-145 cells treated with 50 ng/mL Noco. for 16 h served as a positive control (left). Phosphorylated Cdc25C and Cdc25C protein levels were quantitatively analyzed and compared with GAPDH expression levels using Image J(right). * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001. c Cytoplasmic accumulation of p-Cdc25C (Ser216) in PRRSV-infected MARC-145 cells. Mock- and PRRSV-infected MARC-145 cells at 48 h post-infection were stained for p-Cdc25C (Ser216) (red), F-actin (green), and DNA (blue) with an anti-p-Cdc25C (Ser216) antibody, Phalloidin, and DAPI. Then, the cells were visualized using Leica microsystems (Leica AF6000, Germany) (× 630)

Back to article page