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Fig. 5 | Virology Journal

Fig. 5

From: Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway

Fig. 5

Phosphorylated Cdc2 (Tyr15) expression increases after PRRSV infection. a Detection of Cdc2 and p-Cdc2 (Tyr15) expression with western blot. MARC-145 cells mock-infected and 1 MOI PRRSV-infected were collected 24 h and 48 h after PRRSV infection. Cdc2 and p-Cdc2 (Tyr15) expression was detected with western blot using specific antibodies against Cdc2 or p-Cdc2 (Tyr15). MARC-145 cells treated with 50 ng/mL Noco. for 16 h served as a positive control (left), and their expression levels were quantitatively analyzed and compared with α-tubulin expression using Image J (right). * indicates p < 0.05, ** indicates p < 0.01. b Detection of p-Cdc2 (Tyr15) expression and localization with IFA. Mock- and PRRSV-infected MARC-145 cells at 48 h post-infection were stained with an anti-p-Cdc2 (Tyr15) antibody, Phalloidin, and DAPI to determine p-Cdc2 (Tyr15) (red), F-actin (green), and DNA (blue). Phalloidin was used to show the outline of the cells. Then, the cells were visualized using Leica microsystems (Leica AF6000, Germany) (× 630)

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