Fig. 5

TIP60 colocalized and interacted with NP of IAV during infection. a A549 cells and 293 T cells were transfected with pcDNA3.0-HA-TIP60. At 48 h p.t., the cells were infected with IAV virus (MOI =3). At 12 h.p.i, the cells fixed and stained with DAPI (blue), anti-NP antibody (green) and anti-HA antibody (red). b 293 T cells were transfected with pcDNA3.1-MYC-NP and pcDNA3.0-HA-TIP60 or pcDNA3.0-HA. At 48 h post-transfection, cell lysates were immunoprecipitated with anti-MYC antibody and immunoblotted with anti-MYC or anti-HA antibodies. c A549 cells were infected with H5N1. After 24 h of infection, the cell lysates were subjected to immunoprecipitation with TIP60 antibody following immunoblotting using NP antibody