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Fig. 4 | Virology Journal

Fig. 4

From: Histone acetyl transferase TIP60 inhibits the replication of influenza a virus by activation the TBK1-IRF3 pathway

Fig. 4

Overexpression of TIP60 inhibited Virus replication through activation TBK1-IRF3 signaling Pathway. A549 cells were transfected with empty plasmid pcDNA3.0-HA (Vector) or pcDNA3.0-HA containing TIP60 (TIP60) for 48 h, which were then infected with IAV virus at an MOI of 1 for 12 h. The relative expression level of IL1 (a), IL6 (b), IL12 (c) and IFNβ (d) were determined by qRT-PCR. (e)Total lysate of the infected cells was subjected for Western blot analysis of viral HA-TIP60, p-IRF3, IRF3, p-TBK1, TBK1. β-actin was used as a loading control. Data are mean ± SD of three independent experiments. *p < 0.05; **p < 0.01

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