Fig. 1
IAV upregulated the mRNA and protein expression of TIP60. a Lung epithelial A549 cells were infected with IAV virus (MOI = 1). Cells were harvested 2, 4, 8, and 12 h post-infection and analyzed by western blotting with anti-TIP60 and anti- NP antibodies. b A549 cells were infected with IAV virus at indicated MOIs and harvested at 12-h post infection for Western blot analysis of TIP60, NP and Actin. The TIP60 and Actin protein bands were quantified using Image J software, and the amount of TIP60 was normalized to Actin. c A549 cells (4 × 105) were mock-infected or infected with IAV at an MOI of 1. The uninfected (UNI) and infected (INF) cells were harvested 12 h post-infection and analyzed by qRT-PCR. Data are mean ± SD of three independent experiments. Two-way ANOVA test; *p < 0.05. d 293 T cells were infected with IAV virus (MOI = 1). At 12 h p.i, cells were harvested and analyzed by western blotting