Fig. 4
From: Porcine epidemic diarrhea virus S1 protein is the critical inducer of apoptosis
PEDV infection activates AIFM1, Caspase-3 and Caspase-8. Mock and infected Vero cells were collected at 12, 24, 36, 48, 60 and 72 h post infection and split with RIPA lysis buffer and supersonic. (a-d) Cleaved AIFM1, Caspase-3, Caspase-8 and PARP were analyzed by western blotting. (e-j) The graphs show the quantification of WB bands in panel A-D by testing optical density using ImageJ - system, normalized to β-actin and expressed relative to mock infection. Two biological replicated experiments were performed for each protein, and one result was represented