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Table 2 Accuracy of consensus sequence from serial dilutions (EID50/ml) of NDV LaSota during MinION sequencing run 1

From: Rapid virulence prediction and identification of Newcastle disease virus genotypes using third-generation sequencing

Sequencing run time (min) Total Raw reads 10^6 10^5 10^4 10^3 10^2 10^1
NDV readsa % Identityb NDV reads % Identity NDV reads % Identity NDV reads % Identity NDV reads % Identity NDV reads % Identity
5 4000 283 100 199 100 182 100 10 99.18 2 NA 3 NA
7 8000 644 100 406 100 368 100 14 99.32 8 98.77 5 98.37
10 12,000 1029 100 661 100 571 100 23 99.32 16 99.32 8 99.18
12 16,000 1397 100 901 100 759 100 29 99.86 17 99.32 14 99.45
14 20,000 1772 100 1173 100 1014 100 36 99.73 20 99.45 17 99.59
16 24,000 2183 100 1451 100 1231 100 43 99.73 23 99.45 21 99.59
19 28,000 2643 100 1775 100 1498 100 56 99.86 29 99.73 25 99.45
  1. aThe numbers represent total number of NDV reads obtained from LAclust. A maximum of 200 reads (optional cut-off value) were used to generate full length consensus sequence. Minimum 5 reads were used as a cut-off to build consensus sequence
  2. bConsensus sequence identity to the reference sequence of NDV LaSota sequenced with MiSeq
  3. (MH392212/chicken/USA/LaSota/1946)
  4. Consensus sequences were BLAST searched against NDV custom database
  5. Note: Only 28,000 out of total 60,000 reads were utilized for the analysis