Sequencing run time (min)
|
Total Raw reads
|
10^6
|
10^5
|
10^4
|
10^3
|
10^2
|
10^1
|
---|
NDV readsa
|
% Identityb
|
NDV reads
|
% Identity
|
NDV reads
|
% Identity
|
NDV reads
|
% Identity
|
NDV reads
|
% Identity
|
NDV reads
|
% Identity
|
---|
5
|
4000
|
283
|
100
|
199
|
100
|
182
|
100
|
10
|
99.18
|
2
|
NA
|
3
|
NA
|
7
|
8000
|
644
|
100
|
406
|
100
|
368
|
100
|
14
|
99.32
|
8
|
98.77
|
5
|
98.37
|
10
|
12,000
|
1029
|
100
|
661
|
100
|
571
|
100
|
23
|
99.32
|
16
|
99.32
|
8
|
99.18
|
12
|
16,000
|
1397
|
100
|
901
|
100
|
759
|
100
|
29
|
99.86
|
17
|
99.32
|
14
|
99.45
|
14
|
20,000
|
1772
|
100
|
1173
|
100
|
1014
|
100
|
36
|
99.73
|
20
|
99.45
|
17
|
99.59
|
16
|
24,000
|
2183
|
100
|
1451
|
100
|
1231
|
100
|
43
|
99.73
|
23
|
99.45
|
21
|
99.59
|
19
|
28,000
|
2643
|
100
|
1775
|
100
|
1498
|
100
|
56
|
99.86
|
29
|
99.73
|
25
|
99.45
|
- aThe numbers represent total number of NDV reads obtained from LAclust. A maximum of 200 reads (optional cut-off value) were used to generate full length consensus sequence. Minimum 5 reads were used as a cut-off to build consensus sequence
- bConsensus sequence identity to the reference sequence of NDV LaSota sequenced with MiSeq
- (MH392212/chicken/USA/LaSota/1946)
- Consensus sequences were BLAST searched against NDV custom database
- Note: Only 28,000 out of total 60,000 reads were utilized for the analysis