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Table 3 Overall agreement, lower and upper confidence bound, and the KAPPA value of each flourescent channel per run

From: Validation of intra- and inter-laboratory reproducibility of the Xpert HPV assay according to the international guidelines for cervical cancer screening

 

Agreement

95% CI

Kappa

Kappa 95% CI

Lower bound

Upper bound

Lower bound

Upper bound

Intra-laboratory run 1versusIntra-laboratory run 2

 Fluorescent channel 1

99.4%

98.8%

100%

0.99

97.0%

100%

 Fluorescent channel 2

99.6%

99.1%

100%

0.99

97.8%

100%

 Fluorescent channel 3

98.0%

96.8%

99.2%

0.95

92.4%

98.2%

 Fluorescent channel 4

99.2%

98.5%

100%

0.98

96.3%

100%

 Fluorescent channel 5

98.8%

97.9%

99.8%

0.97

95.0%

99.4%

Intra-laboratory run 1 versusInter-laboratory (Ghent)

 Fluorescent channel 1

99.6%

99.1%

100%

0.99

97.8%

100%

 Fluorescent channel 2

99.6%

99.1%

100%

0.99

97.8%

100%

 Fluorescent channel 3

98.8%

97.9%

99.8%

0.97

94.9%

99.4%

 Fluorescent channel 4

99.2%

98.5%

100%

0.98

96.3%

100%

 Fluorescent channel 5

98.6%

97.6%

99.6%

0.97

94.3%

99.1%

Intra-laboratory run 2 versusInter-laboratory (Ghent)

 Fluorescent channel 1

99.4%

98.8%

100%

0.99

97.0%

100%

 Fluorescent channel 2

100%

100%

100%

1.00

100%

100%

 Fluorescent channel 3

98.8%

97.9%

99.8%

0.97

95.0%

99.4%

 Fluorescent channel 4

99.2%

98.5%

100%

0.98

96.3%

100%

 Fluorescent channel 5

99.8%

99.4%

100%

1.00

98.6%

10%

  1. CI Confidence interval, Fluorescent channel FC 1 detects HPV16, FC2 detects HPV18 and HPV45, FC3 detects HPV31,33,35,52,58, FC4 detects HPV51,59, FC5 detects HPV 39,56,66,68