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Fig. 7 | Virology Journal

Fig. 7

From: Lymphatic endothelial cells promote productive and latent HIV infection in resting CD4+ T cells

Fig. 7

LEC stimulation increases infection rates in activated CD4+ T cells. PBMC were activated with PHA (1 μg/mL) for 3 days before isolating CD4+ T cells by bead depletion. The activated T cells were then cultured alone, with LEC-, or LEC+. All T cells were infected with an HIV reporter virus expressing GFP 1 day after co-culture. a %GFP+ cells were measured 3 days after infection. Samples were taken in triplicates and means+/− standard errors are plotted. Data shown are representative of four independent experiments yielding similar results. Student’s T tests showed significant differences between cells by stimulation. ACT-LEC-: p = 9.99 × 10− 11, ACT-LEC+: p = 6.40 × 10− 9, LEC + -LEC-: 5.36 × 10− 8. b Same experiment as (A). Mean fluorescence intensities of GFP+ cells are shown. Samples were taken in triplicates and means+/− standard errors are plotted. Data shown are representative of four independent experiments yielding similar results. Student’s T tests showed significant differences between cells by stimulation. ACT-LEC-: p = 7.26 × 10− 8, ACT-LEC+: p = 7.71 × 10− 9, LEC + -LEC-: .0015

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