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Fig. 6 | Virology Journal

Fig. 6

From: Lymphatic endothelial cells promote productive and latent HIV infection in resting CD4+ T cells

Fig. 6

Differential involvement of CD2 in HUVEC and LEC stimulation of resting T cells. a CD58 expression in HUVEC (EC) and LEC. EC and LEC were stimulated with or without IFN-γ for 3 days (+/− respectively), and CD58 expressions were measured. b CD2 involvement in HUVEC stimulation of resting CD4+ T cells. Resting CD4+ T cells were cultured alone as a control or with human umbilical vein endothelial cells (EC). One hour before co-culturing with LEC, CD2 blocking antibodies were added at various concentrations (2, 5, and 10 μg/mL) to resting CD4+ T cells. Isotype control antibodies were also included as a negative control. All T cells were infected with an HIV reporter virus expressing GFP 1 day after co-culture. %GFP+ cells were measured on day 6 post-infection. Samples were taken in triplicates and means+/− standard errors are plotted. Data shown are representative of four independent experiments yielding similar results. *Student t-test; p < 0.05 c Similar to b, with both anti-CD2 and anti-IL-6 antibodies. One hour before co-culture, CD2 blocking antibodies (2 μg/mL), IL-6 blocking antibodies (5 μg/mL), and a combination of CD2 blocking antibodies and IL-6 blocking antibodies were added. Isotype control antibodies were also included as a negative control. d CD2 involvement in LEC stimulation of resting CD4+ T cells. Resting T cells were cultured alone, with human lymphatic endothelial cells (LEC). One hour before co-culture, CD2 blocking antibodies, IL-6 blocking antibodies, and a combination of CD2 blocking antibodies and IL-6 blocking antibodies were added at indicated concentrations. Isotype control antibodies were also included as a negative control. All T cells were infected with an HIV reporter virus expressing GFP 1 day after co-culture. %GFP+ cells were measured on day 6 or 7 post-infection. Samples were taken in triplicates and means+/− standard errors are plotted. Data shown are representative of five independent experiments yielding similar results. *Student t-test; p < 0.05

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