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Fig. 5 | Virology Journal

Fig. 5

From: Lymphatic endothelial cells promote productive and latent HIV infection in resting CD4+ T cells

Fig. 5

IL-6 is involved in interactions between human lymphatic endothelial cells and resting CD4+ T cells. a Resting CD4+ T cells were cultured alone or with human lymphatic endothelial cells (LEC). + and - indicate treatment with or without IFN-γ respectively in LEC. At the time of co-culture, anti-human IL-6 antibody was added at various concentrations (5 and 10 μg/mL). Isotype control antibodies were also included as a negative control. All T cells were infected with an HIV reporter virus expressing GFP 1 day after co-culture. %GFP+ cells were measured on day 7 post-infection. Data shown are representative of three independent experiments yielding similar results. Samples were taken in triplicates and means+/− standard errors are plotted. *Student t-test; p < 0.05. b Differential IL-6 levels in HUVEC-stimulated and LEC-stimulated resting T cells. Resting T cells were cultured alone, with human umbilical vein endothelial cells (EC), or human lymphatic endothelial cells (LEC). + and - indicate treatment with or without IFN-γ respectively in EC or LEC. All T cells were infected with an HIV reporter virus expressing GFP 1 day after co-culture, and supernatants were taken for ELISA analysis on day 6 post-infection. Samples were taken in triplicates and means+/− standard errors are plotted. Data shown are representative of four independent experiments yielding similar results

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