Fig. 3

Resting memory T cells are preferentially infected than naïve T cells when co-cultured with LEC. a Infection in naïve and memory T cells stimulated with LEC and HUVEC (EC). Naïve resting T cells and memory resting T cells were each cultured alone, with human umbilical vein endothelial cells (EC), or human lymphatic endothelial cells (LEC). + and - indicate treatment with or without IFN-γ respectively in EC or LEC. All T cells were infected with an HIV reporter virus expressing GFP 1 day after co-culture, and the %GFP+ cells were measured on day 6 post-infection. Samples were taken in triplicates and means+/− standard errors are plotted. Data shown are representative of eight independent experiments yielding similar results. b Mean fluorescence data of the GFP+ cells in the experiment described in (A) are presented. Mean fluorescence intensity data were measured on day 6 post-infection. Samples were taken in triplicates and means+/− standard errors are plotted. Data shown are representative of eight independent experiments yielding similar results. c Activation in naïve and memory T cells stimulated by LEC. On day 6 post-infection, activation levels were examined in T cells by measuring the presence of three activation markers: CD25, HLA-DR, and CD69. d Same experiment as in (c). CD25, HLA-DR, and CD69 levels were individually plotted against GFP expression. Samples were taken in triplicates and means +/− standard errors are plotted. Data shown are representative of four independent experiments yielding similar results