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Table 2 Conservation analysis of amino acids at the cleavage site and receptor-binding sites in the HA protein of the ZJ-1664 and ZJ-1667 H6N1 strains and reference H6 viruses

From: Isolation and characterization of novel reassortant H6N1 avian influenza viruses from chickens in Eastern China

H3 numbering system N-linked glycosylation sites in HA HA cleavage Receptor-binding site Left edge of receptor-binding site Right edge of receptor-binding site
   320–329 98 153 155 183 190 194 195 220–229 134–138
H6 position   339–344 107 166 168 197 204 208 209 234–243 146–150
ZJ-1664(H6N1) 5 PQIETR Y W I H E L Y RPAVNGQRGR GVTKA
ZJ-1667(H6N1) 6 PQIETR Y W I H E L Y RPAVNGQRGR GVTKA
A/chicken/Zhejiang/727031/2014(H6N2) 5 PQIETR Y W V H E L Y RPAVNGQRGR GVSSA
A/duck/Zhejiang/727038/2014(H6N2) 6 PQIETR Y W I H E L Y RPAVNAQRGR GVSSA
A/chicken/Zhejiang/727001/2014(H6N6) 5 PQIETR Y W V H E L Y RPAVNGQRGR GVSSA
A/chicken/Zhejiang/727028/2014(H6N6) 6 PQIETR Y W I H E L Y KPAVNGQRGR GVSSA
A/chicken/Zhejiang/110102/2015(H6N6) 5 PQIETR Y W I H E L Y RPVVNGQRGR GVTSS
A/duck/Hubei/ZYSYG15/2015(H6N2) 6 PQIETR Y W I H E L Y RPAVNGQRGR GVTKA
A/chicken/Hunan/S4191/2009(H6N2) 5 PQIETR Y W V H E L Y RPAVNGQRGR GVSSA
A/duck/Shantou/2195/2003(H6N2) 6 PQIETR Y W I H E L Y RPAVNGQRGR GVSSA
A/wild duck/Shantou/2853/2003(H6N2) 5 PQIETR Y W I H E L Y RPAVNGQRGR GVTSS
A/duck/Hunan/573/2002(H6N2) 5 PQIETR Y W I H E L Y RPAVNGQRGR GVTKA
A/duck/Eastern China/48/2010(H6N6) 5 PQIETR Y W I H E L Y RPAVNGQRGR GVSSA
A/duck/Jiangxi/5178/2007(H6N2) 5 PQIETR Y W I H E L Y RPAVNGQRGR GVSSA
A/duck/Zhejiang/S4204/2010(H6N6) 5 PQIETR Y W I H E L Y RPAVNGQRGR GVSSA
A/swine/Guangdong/K6/2010(H6N6) 5 PQIETR Y W I H E L Y RPVVNGQRSR GVTSS
A/muscovyduck/Vietnam/LBM601/2014(H6N6) 5 PQIETR Y W I H E L Y RPAVNGQRGR GVSSA
A/mallard/Ohio/664/2002(H6N6) 5 PQVKTR Y W I H E L Y RPPANGQRGR GVSSA
A/Teal/Hong Kong/W312/97(H6N1) 6 PQIETR Y W I H E L Y RPAVNGQRGR GTTRS
A/Taiwan/2/2013(H6N1) 6 PQIATR Y W V H V L Y RPAVNGQRSR GVTNA
  1. Residues in bold indicate differences from the consensus alignment