Table 2 Conservation analysis of amino acids at the cleavage site and receptor-binding sites in the HA protein of the ZJ-1664 and ZJ-1667 H6N1 strains and reference H6 viruses

ZJ-1664(H6N1)

PQIETR

Y

W

I

H

E

L

Y

RPAVNGQRGR

GVTKA

ZJ-1667(H6N1)

PQIETR

Y

W

I

H

E

L

Y

RPAVNGQRGR

GVTKA

A/chicken/Zhejiang/727031/2014(H6N2)

PQIETR

Y

W

V

H

E

L

Y

RPAVNGQRGR

GVSSA

A/duck/Zhejiang/727038/2014(H6N2)

PQIETR

Y

W

I

H

E

L

Y

RPAVNAQRGR

GVSSA

A/chicken/Zhejiang/727001/2014(H6N6)

PQIETR

Y

W

V

H

E

L

Y

RPAVNGQRGR

GVSSA

A/chicken/Zhejiang/727028/2014(H6N6)

PQIETR

Y

W

I

H

E

L

Y

KPAVNGQRGR

GVSSA

A/chicken/Zhejiang/110102/2015(H6N6)

PQIETR

Y

W

I

H

E

L

Y

RPVVNGQRGR

GVTSS

A/duck/Hubei/ZYSYG15/2015(H6N2)

PQIETR

Y

W

I

H

E

L

Y

RPAVNGQRGR

GVTKA

A/chicken/Hunan/S4191/2009(H6N2)

PQIETR

Y

W

V

H

E

L

Y

RPAVNGQRGR

GVSSA

A/duck/Shantou/2195/2003(H6N2)

PQIETR

Y

W

I

H

E

L

Y

RPAVNGQRGR

GVSSA

A/wild duck/Shantou/2853/2003(H6N2)

PQIETR

Y

W

I

H

E

L

Y

RPAVNGQRGR

GVTSS

A/duck/Hunan/573/2002(H6N2)

PQIETR

Y

W

I

H

E

L

Y

RPAVNGQRGR

GVTKA

A/duck/Eastern China/48/2010(H6N6)

PQIETR

Y

W

I

H

E

L

Y

RPAVNGQRGR

GVSSA

A/duck/Jiangxi/5178/2007(H6N2)

PQIETR

Y

W

I

H

E

L

Y

RPAVNGQRGR

GVSSA

A/duck/Zhejiang/S4204/2010(H6N6)

PQIETR

Y

W

I

H

E

L

Y

RPAVNGQRGR

GVSSA

A/swine/Guangdong/K6/2010(H6N6)

PQIETR

Y

W

I

H

E

L

Y

RPVVNGQRSR

GVTSS

A/muscovyduck/Vietnam/LBM601/2014(H6N6)

PQIETR

Y

W

I

H

E

L

Y

RPAVNGQRGR

GVSSA

A/mallard/Ohio/664/2002(H6N6)

PQVKTR

Y

W

I

H

E

L

Y

RPPANGQRGR

GVSSA

A/Teal/Hong Kong/W312/97(H6N1)

PQIETR

Y

W

I

H

E

L

Y

RPAVNGQRGR

GTTRS

A/Taiwan/2/2013(H6N1)

PQIATR

Y

W

V

H

V

L

Y

RPAVNGQRSR

GVTNA