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Table 3 Comparison of mOTNRT-PCR and RT-qPCR assays for detecting 398 clinical samples

From: A multiplex one-tube nested real time RT-PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus

Virus No. of samples with result mOTNRT-PCR vs RT-qPCR Performance of mOTNRT-PCR compared with RT-qPCR
+/+ +/− −/+ −/− Sensitivity(%) Specificity(%) Concordance(%)
RSV 95 14 0 289 100 95.38 96.48
HRV 137 13 0 248 100 95.02 96.73
HMPV 38 6 0 354 100 98.33 98.49