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Table 3 Comparison of mOTNRT-PCR and RT-qPCR assays for detecting 398 clinical samples

From: A multiplex one-tube nested real time RT-PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus

Virus

No. of samples with result mOTNRT-PCR vs RT-qPCR

Performance of mOTNRT-PCR compared with RT-qPCR

+/+

+/−

−/+

−/−

Sensitivity(%)

Specificity(%)

Concordance(%)

RSV

95

14

0

289

100

95.38

96.48

HRV

137

13

0

248

100

95.02

96.73

HMPV

38

6

0

354

100

98.33

98.49