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Table 1 Intra-assay of the mOTNRT-PCR

From: A multiplex one-tube nested real time RT-PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus

  Target Conc. (copies/reaction) Number of determinations Mean Ct SD CV (%)
Intra-assay RSV 5 × 106 3 13.23 0.48 3.66
5 × 104 3 19.90 0.13 0.64
5 × 102 3 26.77 0.42 1.57
HRV 5 × 106 3 13.89 0.10 0.74
5 × 104 3 21.60 0.29 1.36
5 × 102 3 27.88 0.14 0.51
HMPV 5 × 106 3 11.83 0.40 3.39
5 × 104 3 19.73 0.26 1.33
5 × 102 3 27.32 0.55 2.01