Target | Primer/probe sequence (5′–3′) | Tm (°C) | Amplicon size (bp) | PCR methodology | |
---|---|---|---|---|---|
HAdV (hexon) | Forward | CAGTGGKCDTACATGCACATC | 55.8 | 75 | qPCR |
Reverse | GCGGGCRAAYTGCACSAG | 60.3 | |||
Probe | FAM- CTCAGGTACTCCGARGC-MGB-NFQ | 53.1 | |||
Internal control | Forward | GTTCACCGATAGACCGCTG | 55.5 | 113 | qPCR |
Reverse | AAGAGCCCGGAATGTCAAGA | 56.3 | |||
Probe | Cy5-ACTACCTGAGCACCCAGTCCGCCCT-BBQ | 67.3 | |||
HAdV (hexon) | Forward | GCCACCTTYTTCCCCATGGC | 60.5 | 1004 | Conventional PCR (1st round) |
Reverse | GTAGCGTTRCCGGCNGAGAA | 59.8 | |||
Forward | TTCCCCATGGCNCACAACAC | 59.6 | 956 | Conventional PCR (nested); partial hexon gene sequencing | |
Reverse | GCCTCRATGACGCCGCGGTG | 65.2 |