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Table 4 Primers and probes used in the present study

From: Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus

Target

Primer/probe sequence (5′–3′)

Tm (°C)

Amplicon size (bp)

PCR methodology

HAdV (hexon)

Forward

CAGTGGKCDTACATGCACATC

55.8

75

qPCR

Reverse

GCGGGCRAAYTGCACSAG

60.3

Probe

FAM- CTCAGGTACTCCGARGC-MGB-NFQ

53.1

Internal control

Forward

GTTCACCGATAGACCGCTG

55.5

113

qPCR

Reverse

AAGAGCCCGGAATGTCAAGA

56.3

Probe

Cy5-ACTACCTGAGCACCCAGTCCGCCCT-BBQ

67.3

HAdV (hexon)

Forward

GCCACCTTYTTCCCCATGGC

60.5

1004

Conventional PCR (1st round)

Reverse

GTAGCGTTRCCGGCNGAGAA

59.8

 

Forward

TTCCCCATGGCNCACAACAC

59.6

956

Conventional PCR (nested); partial hexon gene sequencing

Reverse

GCCTCRATGACGCCGCGGTG

65.2

  1. bp base pairs, Tm melting temperature