Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus

Table 2 Replication experiment to evaluate precision for plasma (A) and VTM (B)

Nominal (log₁₀ copies/mL)	No. of sample tested	Mean HAdV DNA load (log₁₀ copies/mL) Intra-assay	SD (log₁₀ copies/mL) Intra-assay	%CV Intra-assay	Mean HAdV DNA load (log₁₀ copies/ ml) Inter-assay	SD (log₁₀ copies/mL) Inter-assay	%CV Inter-assay
A
9	3	9.12	0.01	0.05	9.12	0.01	0.07
8	3	8.07	0.01	0.09	8.07	0.01	0.07
7	3	6.94	0.01	0.13	6.94	0.01	0.10
6	3	5.82	0.01	0.11	6.81	0.01	0.12
5	3	4.80	0.02	0.38	4.80	0.02	0.33
4	3	4.08	0.02	0.54	4.10	0.03	0.71
3	3	3.16	0.05	1.50	3.15	0.10	3.21
2.60	3	2.85	0.02	0.63	2.90	0.07	2.42
B
9	3	9.11	0.01	0.05	9.12	0.02	0.17
8	3	8.03	0.01	0.10	8.02	0.01	0.17
7	3	6.93	0.01	0.22	6.92	0.02	0.26
6	3	5.87	0.01	0.19	5.88	0.02	0.26
5	3	4.91	0.02	0.37	4.91	0.01	0.25
4	3	4.05	0.05	1.27	4.05	0.04	1.00
3	3	3.10	0.06	1.84	3.10	0.04	1.44
2.94	3	2.92	0.02	0.57	2.92	0.06	2.11

ISSN: 1743-422X