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Table 2 Replication experiment to evaluate precision for plasma (A) and VTM (B)

From: Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus

Nominal (log10 copies/mL) No. of sample tested Mean HAdV DNA load (log10 copies/mL) Intra-assay SD (log10 copies/mL) Intra-assay %CV Intra-assay Mean HAdV DNA load (log10 copies/ ml) Inter-assay SD (log10 copies/mL) Inter-assay %CV Inter-assay
A
 9 3 9.12 0.01 0.05 9.12 0.01 0.07
 8 3 8.07 0.01 0.09 8.07 0.01 0.07
 7 3 6.94 0.01 0.13 6.94 0.01 0.10
 6 3 5.82 0.01 0.11 6.81 0.01 0.12
 5 3 4.80 0.02 0.38 4.80 0.02 0.33
 4 3 4.08 0.02 0.54 4.10 0.03 0.71
 3 3 3.16 0.05 1.50 3.15 0.10 3.21
 2.60 3 2.85 0.02 0.63 2.90 0.07 2.42
B        
 9 3 9.11 0.01 0.05 9.12 0.02 0.17
 8 3 8.03 0.01 0.10 8.02 0.01 0.17
 7 3 6.93 0.01 0.22 6.92 0.02 0.26
 6 3 5.87 0.01 0.19 5.88 0.02 0.26
 5 3 4.91 0.02 0.37 4.91 0.01 0.25
 4 3 4.05 0.05 1.27 4.05 0.04 1.00
 3 3 3.10 0.06 1.84 3.10 0.04 1.44
 2.94 3 2.92 0.02 0.57 2.92 0.06 2.11