Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus

Table 1 PCR results used for the calculation of the analytical sensitivity

Sample matrix	Input concentration (log₁₀ copies/mL)	Number of replicates	Number of positives	Hit rates (%)
Plasma	2.90	16	16	100
	2.60	16	16	100
	2.30	16	16	100
	2.00	16	15	93.75
	1.70	16	8	50
	1.40	16	3	18.75
	No plasmid DNA	16	0	0
Viral transport medium	2.94	16	16	100
	2.64	16	16	100
	2.34	16	16	100
	2.04	16	11	68.75
	1.74	16	5	31.25
	1.44	16	2	12.5
	No plasmid DNA	16	0	0

ISSN: 1743-422X