Fig. 6

Cellular co-localization of plasma membrane with M1 and M165A mutant using immunofluorescence confocal microscopy. Co-cultured 293 T and MDCK cells were transfected with eight plasmids for reverse genetic system. After 24 h, the cells were fixed with methanol, plasmatic membrane were labelled with anti-sodium potassium ATPase antibody-plasma membrane marker (Alexa Fluor 488) (in green). M1 and M165A were detected using a primary anti-M1 antibody and a secondary antibody coupled to Alexa555 (in red). Nuclei were stained with DAPI (in blue). NC represents non transfected cells, PMM represents plasma membrane. The plasma membrane and intracellular distributions of M1 were imaged by confocal laser scanning fluorescence microscopy (LSM Zeiss 510 Meta). Insets show higher magnification views of the selected areas. The results are representative of three independent experiments