Fig. 5

Cellular co-localization of CRM1 with M1 and M165A mutant using immunofluorescence confocal microscopy. Co-cultured 293 T and MDCK cells were transfected with eight plasmids for reverse genetic system. After 24 h, the cells were fixed and M1 and M165A were detected using a primary anti-M1 antibody and a secondary antibody coupled to Alexa488 (in green). CRM1 protein was labelled with anti-CRM1 antibody and a secondary antibody coupled with Alexa555 (in red). Nuclei were stained with DAPI (in blue). NC represents non transfected cells. The intracellular distributions of M1 and CRM1 were imaged by confocal laser scanning fluorescence microscopy (LSM Zeiss 510 Meta). Insets show higher magnification views of the selected areas. The results are representative of three independent experiments