Fig. 3From: A loop-mediated isothermal amplification assay for the detection and quantification of JC polyomavirus in cerebrospinal fluid: a diagnostic and clinical management tool and technique for progressive multifocal leukoencephalopathyAmplification of the standard DNA in the LAMP assay. a Serially fivefold dilutions of standard DNA (from 1.0 × 100 to 2.0 × 106 copies/reaction) were subjected to the assay, and the LAMP products were monitored in real-time with a turbidimeter. Standard diluent was used as a negative control (NC). b A standard curve for the quantitative LAMP assay. The time to a positive result (Tp) was plotted against input standard DNA (from 1.3 × 102 to 2.0 × 106 copies/reaction). The results are the average of four replicates. Error bars represent the standard deviation (SD)Back to article page