Fig. 8

Treatment with ι-carrageenan or heparin lyase selectively inhibits basolateral EBOV binding. a EBOV was pretreated with media or 20 ng/μl of ι-carrageenan and added to Caco-2 cells at a concentration of 3 pfu/μl at 4 °C for particle binding. Cells were washed 3X with ice cold PBS to remove excess virus and lysed with TRIzol. b Caco-2 cells were treated with a Heparin lyase I and III blend for 1 h before infection. The cells were then washed with sterile PBS chilled to 4 °C and infected with EBOV either apically or basolaterally at the same concentration and incubated at 4 °C for particle binding. Cells were washed 3X with ice cold PBS to remove excess virus and lysed with TRIzol. EBOV RNA expression was measured at 24 hpi, using SYBR-green qPCR assay and normalized to GAPDH expression. Fold change results are expressed in mean ± SD calculated from three independent experiments. Data was analyzed using one-way ANOVA n.s. > 0.05, *p < 0.05, **p < 0.01