Fig. 6
From: Heparan sulfate is an important mediator of Ebola virus infection in polarized epithelial cells
Mechanical damage to Caco-2 monolayer increases susceptibility to apical infection. Caco-2 monolayers were scratched with a pipette tip across the apical surface to expose the underlying basal cells along the injury path (yellow dashed lines). The apical surfaces of injured cultures were immediately infected with EBOV at 3 pfu/cell. At 24 hpi, the cultures were fixed and immunostained with antibody to EBOV-GP, and E-cadherin, and the cultures were examined by fluorescence microscopy. Original magnification 40X