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Fig. 1 | Virology Journal

Fig. 1

From: Heparan sulfate is an important mediator of Ebola virus infection in polarized epithelial cells

Fig. 1

Establishment of a polarized Caco-2 cell monolayer. a Caco-2 monolayers were seeded at a density of 4 × 104 and allowed to grow for 10 days after seeding. TEER readings were taken every other day and normalized to resistance of unseeded well taken at the same time point. Values plotted are mean ± SD calculated from three independent experiments. b Caco-2 cells were grown for 6 days after seeding on semipermeable membranes and then fixed with 10% PBS buffered formalin (E-cadherin) or ice cold methanol (ZO-1) and examined by immunofluorescence microscopy

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